中部大学

中部大学

教員情報

岩崎 雄吾 IWASAKI Yugo

プロフィール

生年 1968
職名 教授
所属 応用生物学部 応用生物化学科
大学院 応用生物学研究科 応用生物学専攻
最終学歴 名古屋大学大学院農学研究科食品工業化学専攻博士課程後期課程中退
学位 博士(農学)(名古屋大学)
専門分野 酵素工学および脂質化学
研究テーマ 酵素、微生物を利用した有用脂質類の合成研究
授業科目 基礎化学、微生物学II、応用微生物学、遺伝子工学、食品衛生学

著書および訳書

岩崎雄吾(分担)「3)脂質,テルペノイド」(第5章 微生物の代謝),応用微生物学 第3版(横田 篤, 大西康夫, 小川 順 編)文永堂出版128–131,(2016)

山根 恒夫,中野 秀雄, 加藤 雅士, 岩崎 雄吾, 河原崎 泰昌, 志水 元亨: 新版 生物反応工学. 産業図書(2016)

学術論文、評論

原著論文

Yicheng Wang, Anant K. Menon, Yuta Maki, Yi-Shi Liu, Yugo Iwasaki, Morihisa Fujita, Paula A. Guerrero, Daniel Varón Silva, Peter H. Seeberger, Yoshiko Murakami, Taroh Kinoshita: Genome-wide CRISPR screen reveals CLPTM1L as a lipid scramblase required for efficient glycosylphosphatidylinositol biosynthesis. Proc. Nat. Acad Sci, 119, e2115083119 (2022).

Hiroyuki Ashida, Kaho Murakami, Kenji Inagaki, Yoshihiro Sawa, Hisashi Hemmi, Yugo Iwasaki, Tohru Yoshimura: Evolution and properties of alanine racemase from Synechocystis sp. PCC6803. J. Biochem. 171, 421-428 (2021).

Yicheng Wang, Anant K. Menon, Yuta Maki, Yi-Shi Liu, Yugo Iwasaki, Morihisa Fujita, Paula A. Guerrero, Daniel Varón Silva, Peter H. Seeberger, Yoshiko Murakami, Taroh Kinoshita: CLPTM1L is a lipid scramblase involved in glycosylphosphatidylinositol biosynthesis. bioRxiv preprint doi: https://doi.org/10.1101/2021.07.12.451801; (2021). Preprint.

Ariela Samantha, Jasmina Damnjanović, Yugo Iwasaki, Hideo Nakano, Alice Vrielink: Structures of an engineered phospholipase D with specificity for secondary alcohol transphosphatidylation: Insights into plasticity of substrate binding and activation. Biochem. J. 478, 1749-1767 (2021).

Yugo Iwasaki, Yuuki Sakurai and Jasmina Damnjanović: A simple chemo-enzymatic synthesis of alkyl-acyl (plasmanyl) phospholipids. Biocatal. Agric. Biotechnol. 26, 101624 (2020).

Jasmina Damnjanović, Hideo Nakano and Yugo Iwasaki: Acyl chain that matters: Introducing sn-2 acyl chain preference to a phospholipase D by protein engineering. Protein Eng. Des. Sel. 32, 1-11, (2019).

Alfi A., Zhu, Bou, Jasmina Damnjanović, Takaaki Kojima, Yugo Iwasaki, and Hideo Nakano : Production of active Manganese peroxidase in Escherichia coli by co-expression of chaperones and in vitro maturation by ATP-dependent chaperone release. J. Biosci. Bioeng. 128, 290-295, (2019)

Lei Wang, Yugo Iwasaki, Kiran K. Andra, Kalpana Pandey, Anant K. Menon and Peter Bütikofer: Scrambling of natural and fluorescently tagged phosphatidylinositol by reconstituted G protein–coupled receptor and TMEM16 scramblases. J. Biol. Chem. 293, 18318-18327 (2018).

Jasmina Damnjanović, Nozomi Matsunaga, Masaatsu Adachi, Hideo Nakano and Yugo Iwasaki: Facile enzymatic synthesis of phosphatidylthreonine using an engineered phospholipase D. Eur. J. Lipid Sci. Technol. 120, 1800089, (2018).

Arisa Inoue, Masaatsu Adachi, Jasmina Damnjanović, Hideo Nakano and Yugo Iwasaki: Direct enzymatic synthesis of 1-phosphatidyl-β-D-glucose by engineered phospholipase D. ChemistrySelect, 1, 4121–4125 (2016).

Michiko Muraki, Jasmina Damnjanović, Hideo Nakano and Yugo Iwasaki: Salt-induced increase in the yield of enzymatically synthesized phosphatidylinositol and the underlying mechanism. J. Biosci. Bioeng., 122, 276–282 (2016).

Eiji Omoto, Yugo Iwasaki, Hiroshi Miyake, and Mitsutaka Taniguchi: Salinity induces membrane structure and lipid changes in maize mesophyll and bundle sheath chloroplasts. Physiol. Plant. 157, 13-23 (2016).

Ayana Ujiie, Hideo Nakano and Yugo Iwasaki: Extracellular production of Pseudozyma (Candida) antarctica lipase B with genuine primary sequence in recombinant Escherichia coli. J. Biosci. Bioeng. 121, 303–309 (2016).

Jasmina Damnjanović, Chisato Kuroiwa, Hidetoshi Tanaka, Ken Ishida, Hideo Nakano and Yugo Iwasaki: Directing positional specificity in enzymatic synthesis of bioactive 1-phosphatidylinositol by protein engineering of a phospholipase D. Biotechnol. Bioeng. 113, 62–71 (2016).

Akihiro Mori, Shoichi Hara, Tomohiro Sugahara, Takaaki Kojima, Yugo Iwasaki, Yasuaki Kawarasaki, Takehiko Sahara, Satoru Ohgiya, and Hideo Nakano: Signal peptide optimization tool for the secretion of recombinant protein from Saccharomyces cerevisiae. J. Biosci. Bioeng. 120, 510–525 (2015).

Jasmina Damnjanović, Hideo Nakano and Yugo Iwasaki: Deletion of a dynamic surface loop improves stability and changes kinetic behavior of phosphatidylinositol-synthesizing Streptomyces phospholipase D. Biotechnol. Bioeng., 112, 674–682 (2014).

Chisato Eba, Aoi Okano, Hideo Nakano and Yugo Iwasaki: A chromogenic substrate for solid-phase detection of phospholipase A2. Anal. Biochem., 447, 43–45 (2014).

Ryoko Ninomiya, Bo Zhu., Takaaki Kojima, Yugo Iwasaki, and Hideo Nakano: Role of disulfide bond isomerase DsbC, calcium ions, and hemin in cell-free protein synthesis of active manganese peroxidase isolated from Phanerochaete chrysosporium. J. Biosci. Bioeng., 117, 652–657 (2014).

Yusuke Wada, Atsuki Matsubara, Takato Uchikata, Yugo Iwasaki, Satoshi Morimoto, Katsuta Kan, Tetsuya Ookura, Eiichiro Fukusaki, Takeshi Bamba: Investigation of β-Cryptoxanthin Fatty Acid Ester Compositions in Citrus Fruits Cultivated in Japan. Food Nutr. Sci., 4, 98–104 (2013).

Jasmina Damnjanović, Hideo Nakano and Yugo Iwasaki: Simple and efficient profiling of phospholipids in phospholipase D-modified soy lecithin by HPLC with charged aerosol detection. J. Am. Oil Chem. Soc., 90, 951–957 (2013).

Jasmina Damnjanović, Rie Takahashi, Atsuo Suzuki, Hideo Nakano and Yugo Iwasaki: Improving thermostability of phosphatidylinositol-synthesizing Streptomyces phospholipase D. Protein Eng. Des. Sel., 25, 415–424 (2012).

Daiki Takemori, Kenta Yoshino, Chisato Eba, Hideo Nakano and Yugo Iwasaki: Extracellular production of phospholipase A2 from Streptomyces violaceoruber by recombinant Escherichia coli. Protein Expr. Purif., 81, 145–150 (2012).

Yusuke Wada, Atsuki Matsubara, Takato Uchikata, Yugo Iwasaki, Satoshi Morimoto, Katsuta Kan, Tetsuya Okura, Eiichiro Fukusaki and Takeshi Bamba: Metabolic profiling of ß-cryptoxanthin and its fatty acid esters by supercritical fluid chromatography coupled with triple quadrupole mass spectrometry. J. Sep. Sci., 34, 3546–3552 (2011).

Tatsushi Tanaka, Tetsuo Isezaki, Hideo Nakano and Yugo Iwasaki: Synthesis of phospholipids containing polyunsaturated fatty acids by phospholipase A2-mediated esterification with food-compatible reagents. J. Oleo Sci., 59, 375–380 (2010).

Akari Ozaki, Atsushi Masayama, Hideo Nakano and Yugo Iwasaki: Synthesis of phosphatidylinositols having various inositol stereoisomers by engineered phospholipase D. J. Biosci. Bioeng., 109, 337–340 (2010).

Yugo Iwasaki, Atsushi Masayama, Akihiro Mori, Chika Ikeda and Hideo Nakano: Composition analysis of positional isomers of phosphatidylinositol by high-performance liquid chromatography. J. Chromatogr. A. 1216, 6077–6080 (2009).

Atsushi Masayama, Kaori Tsukada, Chika Ikeda, Hideo Nakano and Yugo Iwasaki: Isolation of phospholipase D mutants having phosphatidylinositol-synthesizing activity with positional specificity on myo-inositol. ChemBioChem, 10, 559–564 (2009).

Li Deng, Hideo Nakano and Yugo Iwasaki: Direct separation of monoacylglycerol isomers by enantioselective high-performance liquid chromatography. J. Chromatogr. A, 1198-1199C, 67–72 (2008).

Atsushi Masayama, Tetsuya Takahashi, Kaori Tsukada, Seigo Nishikawa, Rie Takahashi, Masaatsu Adachi, Kazushi Koga, Atsuo Suzuki, Takashi Yamane, Hideo Nakano, and Yugo Iwasaki: Streptomyces phospholipase D mutants with altered substrate specificity capable of phosphatidylinositol synthesis. ChemBioChem, 9, 974–981 (2008).

Li Deng, Hideo Nakano and Yugo Iwasaki: Direct separation of regioisomers and enantiomers of monoacylglycerols by tandem column high-performance liquid chromatography. J. Chromatogr. A, 1165, 93–99 (2007).

Weera Piyatheerawong, Tsuneo Yamane, Hideo Nakano and Yugo Iwasaki: Enzymatic preparation of enantiomerically pure sn-2,3-diacylglycerols: A stereoselective ethanolysis approach. J. Am. Oil Chem. Soc., 83, 603–607 (2006).

Weera Piyatheerawong, Yugo Iwasaki, and Tsuneo Yamane: Direct separation of regio- and enantiomeric isomers of diacylglycerols by a tandem column high-performance liquid chromatography. J. Chromatogr. A, 1068, 243–248 (2005).

Yugo Iwasaki, Seigo Nishikawa, Maki Tsuneda, Tetsuya Takahashi, and Tsuneo Yamane: Detection of phospholipase D on solid materials. Anal. Biochem., 329, 157–159 (2004).

Weera Piyatheerawong, Yugo Iwasaki, Xuebing Xu, and Tsuneo Yamane: Dependency of water concentration on ethanolysis of trioleoylglycerol by lipases. J. Mol.Catal. B Enzym., 28, 19–24 (2004).

Yugo Iwasaki, Yukiko Mizumoto, Takahiro Okada, Takaya Yamamoto, Kentaro Tsutsumi and Tsuneo Yamane: An aqueous suspension system for phospholipase D-mediated synthesis of phosphatidylserine without toxic organic solvent. J. Am. Oil Chem. Soc., 80, 653–657 (2003).

S. Shanmuga Sundara Raj, Eiji Kibushi, Tatsuhiro Kurasawa, Atsuo Suzuki, Takashi Yamane, Shoji Odani, Yugo Iwasaki, Tsuneo Yamane, and Tamaichi Ashida: Crystal structure of bovine trypsin and wheat germ trypsin inhibitor (I-2b) complex at 2.3 Å resolution. J. Biochem., 132, 927–933 (2002).

Roxana Irimescu, Yugo Iwasaki, and Ching T. Hou: Study of TAG ethanolysis to 2-MAG by immobilized Candida antarctica lipase and synthesis of symmetrically structured TAG. J. Am. Oil Chem. Soc., 79, 879–883 (2002).

Yugo Iwasaki, Wanda Brown and Ching T. Hou: Biosynthetic pathway of diepoxy bicyclic fatty acids from linoleic acid by Clavibacter sp. ALA2. J. Am. Oil Chem.Soc., 79, 369–372 (2002).

Xiuping Jiang, Kazuyo Oohira, Yugo Iwasaki, Hideo Nakano, Shigeyuki Ichihara and Tsuneo Yamane: Reduction of protein degradation by use of protease-deficient mutants in cell-free protein synthesis system of Escherichia coli. J. Biosci. Bioeng., 93, 151–156, (2002).

Roxana Irimescu, Kiyomi Furihata, Kazuhiko Hata, Yugo Iwasaki, and Tsuneo Yamane: Two-step enzymatic synthesis of docosahexaenoic acid-rich symmetrically structured triacylglycerol via 2-monoacylglycerols. J. Am. Oil Chem. Soc., 78, 743–748 (2001).

Roxana Irimescu, Kiyomi Furihata, Kazuhiko Hata, Yugo Iwasaki, and Tsuneo Yamane: Utilization of reaction medium-dependent regiospecificity of Novozym 435 for synthesis of 1,3-dicapryloyl-2-docosahexaenoyl (or eicosapentaenoyl) glycerol. J. Am. Oil Chem. Soc., 78, 285–289 (2001).

Roxana Irimescu, Kazuhiko Hata, Yugo Iwasaki, and Tsuneo Yamane: Comparison of acyl donors for lipase-catalyzed production of 1,3-dicapryloyl-2-eicosapentaenoylglycerol. J. Am. Oil Chem. Soc., 78, 65–70 (2001).

Yugo Iwasaki, Mamoru Yasui, Takuma Ishikawa, Roxana Irimescu, Kazuhiko Hata and Tsuneo Yamane: Optical resolution of asymmetric triacylglycerols by chiral-phase high performance liquid chromatography. J. Chromatogr. A, 905, 111–118 (2001).

Jiann-Tsyh Lin, Karen M. Lew, Jennifer M. Chen, Yugo Iwasaki and Thomas A. McKeon: Metabolism of 1-acyl-2-oleoyl-sn-glycero-3-phosphoethanolamine in castor oil biosynthesis Lipids, 35, 481–486 (2000).

Yugo Iwasaki, Tatsuaki Nishiyama, Yasuaki Kawarasaki, Hideo Nakano and Tsuneo Yamane: Importance of disulfide bridge formation on folding of phospholipase D from Streptomyces antibioticus. J. Biosci. Bioeng., 89, 506–508 (2000).

Jun-Hao Yang, Koei Kobayashi, Yugo Iwasaki, Hideo Nakano and Tsuneo Yamane: In vitro analysis of roles of a disulfide bridge and a calcium binding site in activation of Pseudomonas sp. strain KWI-56 lipase. J. Bacteriol., 182, 295–302 (2000).

Roxana Irimescu, Mamoru Yasui, Yugo Iwasaki, Nobuyoshi Shimidzu and Tsuneo Yamane: Enzymatic synthesis of 1,3-dicapryloyl-2-eicosapentaenoylglycerol. J. Am. Oil Chem. Soc., 77, 501–506 (2000).

Yugo Iwasaki, Satoko Horiike, Kayoko Matsushima and Tsuneo Yamane: Location of the catalytic nucleophile of phospholipase D of Streptomyces antibioticus in the C-terminal half domain. Eur. J. Biochem., 264, 577–581 (1999).

Roxana Rosu, Mamoru Yasui, Yugo Iwasaki, and Tsuneo Yamane: Enzymatic synthesis of symmetrical 1,3-diacylglycerols by direct esterification of glycerol in solvent-free system. J. Am. Oil Chem. Soc., 76, 839–843 (1999).

Yugo Iwasaki, Jeong Jun Han, Miho Narita, Roxana Rosu and Tsuneo Yamane: Enzymatic synthesis of structured lipids from single cell oil of high docosahexaenoic acid content. J. Am. Oil Chem. Soc., 76, 563–569 (1999).

Jeong-Jun Han, Yugo Iwasaki and Tsuneo Yamane: Use of isopropanol as a modifier in a hexane-acetonitrile based mobile phase for the silver ion HPLC separation of positional isomers of triacylglycerols containing long chain polyunsaturated fatty acids. J. High Resol. Chromatogr., 22, 357–361 (1999).

Atsuo Suzuki, Kouji Kakuno, Yugo Iwasaski, Tsuneo Yamane and Takashi Yamane: Crystallization and preliminary X-ray crystallographic studies of phospholipase D from Streptomyces antibioticus. Acta Crystarogr. D, 55, 317–319 (1999).

Jeong-Jun Han, Yugo Iwasaki and Tsuneo Yamane: Monitoring of lipase-catalyzed transesterification between eicosapentaenoic acid ethylester and tricaprylin by silver ion high performance liquid chromatography and high temperature gas chromatography. J. Am. Oil Chem. Soc., 76, 31–39 (1999).

Roxana Rosu, Yugo Iwasaki and Tsuneo Yamane: Intensification of lipase performance in a transesterification reaction by immobilization on CaCO3 powder. J. Biotechnol., 66, 51–59 (1998).

Roxana Rosu, Yugo Iwasaki, Nobuyoshi Shimidzu, Nobushige Doisaki and Tsuneo Yamane: Enzymatic synthesis of glycerides from DHA-enriched PUFA ethyl ester by glycerolysis under vacuum. J. Mol. Catal. B Enzym., 4, 191–198 (1998).

Yugo Iwasaki, Yukiko Tsubouchi, Akiko Ichihashi, Hideo Nakano, Tomoko Kobayashi, Hiroh Ikezawa and Tsuneo Yamane: Two distinct phosphatidylinositol-specific phospholipase C from Streptomyces antibioticus. Biochim. Biophys. Acta, 1391, 52–66 (1998).

Naoto Mishima, Kousaku Mizumoto, Yugo Iwasaki, Hideo Nakano and Tsuneo Yamane: Insertion of stabilizing loci in vectors of T7 RNA polymerase-mediated Escherichia coli expression systems: A case study on the plasmids involving foreign phospholipase D gene. Biotechnol. Prog., 13, 864–868, (1997).

Roxana Rosu, Yuki Uozaki, Yugo Iwasaki and Tsuneo Yamane: Repeated use of immobilized lipase for monoacylglycerol production by solid-phase glycerolysis of olive oil. J. Am. Oil Chem. Soc., 74, 445–450 (1997).

Yugo Iwasaki, Naoto Mishima, Kousaku Mizumoto, Hideo Nakano and Tsuneo Yamane: Extracellular production of phospholipase D of Streptomyces antibioticus using recombinant Escherichia coli. J. Ferment. Bioeng., 79, 417–421 (1995).

Yugo Iwasaki, Shinobu Niwa, Hideo Nakano, Toru Nagasawa and Tsuneo Yamane: Purification and properties of phosphatidylinositol-specific phospholipase C from Streptomyces antibioticus. Biochim. Biophys. Acta, 1214, 221–228 (1994).

Yugo Iwasaki, Hideo Nakano and Tsuneo Yamane: Phospholipase D gene from Streptomyces antibioticus: cloning, sequencing, expression and relationship to other phospholipases. Appl. Microbiol. Biotechnol., 42, 290–299 (1994).

Kikuo Shimbo, Yugo Iwasaki, Tsuneo Yamane and Kazuo Ina: Purification and properties of phospholipase D from Streptomyces antibioticus. Biosci. Biotech. Biochem., 57, 1946–1948 (1993).

総説・解説

岩崎雄吾, Jasmina Damnjanović: 改変型ホスホリパーゼDによる難合成性リン脂質の合成 生物工学 98, 9, 481-484(2020).

Jasmina Damnjanović、岩崎雄吾:生物工学分野における分子ドッキングシミュレーションの応用 生物工学 97, 5, 257-260(2019).

Jasmina Damnjanović and Yugo Iwasaki: Chapter 3, Enzymatic Modification of Phospholipids in "Recent Advances in Biotechnology Vol. 4. Progress in Food Biotechnology" (edited by Ali Osman). Bentham Science, pp. 82-131 (2018).

Jasmina Damnjanović and Yugo Iwasaki: Chapter 4, Enzymatic Modification of Phospholipids by Phospholipase D in "Lipid Modification by Enzymes and Engineered Microbes" (Edited by U.T. Bornscheuer), AOCS press, pp 69-88 (2018).

岩崎雄吾: 放線菌ホスホリパーゼDの改変とリン脂質合成への応用 オレオサイエンス 13, 465-469 (2013).

Jasmina Damnjanović and Yugo Iwasaki: Phospholipase D as a catalyst: application in phospholipid synthesis, molecular structure and protein engineering. J. Biosci. Bioeng. 116, 271-280 (2013).

昌山敦,鈴木淳巨,岩崎雄吾,ホスホリパーゼDの機能改変 生物工学会誌 87, 274-276 (2009)

岩崎雄吾, 昌山敦, 中野秀雄, ホスホリパーゼDによるリン脂質の変換 微生物によるものづくり 監修:植田充美 シーエムシー出版 pp100-105 (2008)

岩崎雄吾, 山根恒夫 ホスホリパーゼDの酵素工学 生物工学会誌 82, 297-299 (2004)

Yugo Iwasaki and Tsuneo Yamane: Enzymatic synthesis of structured lipids. Adv. Biochem. Eng. Biotechnol. 90, 151-171 (2004).

岩崎雄吾, 山根恒夫: 構造脂質の酵素合成 オレオサイエンス, 1, 825-833 (2001).

Yugo Iwasaki and Tsuneo Yamane: Phospholipases in Enzyme Engineering of Phospholipids for Food, Cosmetics and Medical Applications in Lipid Biotechnology, (T.M. Kuo and H.W. Gardner ed.), Chap.20, pp417-431, Marcel Deckker Inc. New York (2002).

山根恒夫、岩崎雄吾: 機能性脂質とバイオテクノロジー バイオサイエンスとバイオインダストリー, 58, 11-16 (2000).

Yugo Iwasaki and Tsuneo Yamane.: Enzyme Synthesis of Structured lipids. J. Mol.Catalysis B: Enzymatic, 10, (1-3), 129-140 (2000).

Tsuneo Yamane, Yugo Iwasaki, Roxana Rosu, Nobuyoshi Shimidzu and Nobushige Doisaki: Multiple intensified performance of an enzyme-catalyzed reaction in organic media. Annals. NY Acad. Sci., 864, 171-179 (1998)

山根恒夫、岩崎雄吾: リン脂質類の酵素変換 油化学、44, 875-882 (1995).

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